Question 1

When preparing to isolate proteins from plant cells, the first step in preparing the cell homogenate would be

Accepted Answer

A)   sonication.
B)   using a French press.
C)   treatment with mild detergents.
D)   enzymatic treatment.E) C) and D)
F) None of the above

Question 2

Why is X-ray crystallography more commonly used than NMR spectroscopy when determining protein structure?

Accepted Answer

NMR is limited to proteins that are less...

Question 3

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions. Which fraction corresponds to the cytosol fraction?

Accepted Answer

A)   A
B)   B
C)   C
D)   DE) A) and C)
F) A) and B)

Question 4

In an ELISA, the capture antibody is a antibody.

Accepted Answer

A)   monoclonal primary
B)   polyclonal primary
C)   monoclonal secondary
D)   polyclonal secondaryE) C) and D)
F) All of the above

Question 5

How does NMR spectroscopy differ from X-ray crystallography when determining the structure of a protein?

Accepted Answer

X-ray crystallography gives the atomic p...

Question 6

After centrifugation, the purity of the protein is determined by

Accepted Answer

A)   absorbance measurements.
B)   activity units.
C)   total protein content.
D)   specific activity.E) A) and C)
F) All of the above

Question 7

Explain how protein samples that contain the antigenic protein are recognized using a Western blot.

Accepted Answer

The proteins are separated using gel ele...

Question 8

In monoclonal antibody generation, the cells that produce the antibody in the animal are located in cells.

Accepted Answer

A)   heart
B)   liver
C)   spleen
D)   red bloodE) None of the above
F) All of the above

Question 9

Why is protein purification often referred to as an art and a science?

Accepted Answer

There are between 10,000 and 100,000 dif...

Question 10

What is the advantage of using polyclonal antibodies compared with monoclonal antibodies?

Accepted Answer

A)   They are less labor intensive to generate.
B)   They provide the ability to monitor quality continually.
C)   The process used to produce the antibodies provides unlimited supplies.
D)   They can be generated without using animals.E) B) and C)
F) A) and D)

Question 11

How does the directionality of synthesis differ with in vivo polypeptide synthesis compared to solid-phase peptide synthesis?

Accepted Answer

In in vivo polypeptide synthesis, amino ...

Question 12

In isoelectric focusing, a protein with a pH below the pI would

Accepted Answer

A)   migrate toward the anode.
B)   migrate toward the cathode.
C)   stop migrating.
D)   migrate, but there is no way to determine the direction.E) None of the above
F) C) and D)

Question 13

What is the advantage of using monoclonal antibodies compared with polyclonal antibodies?

Accepted Answer

A)   They are less labor intensive to generate.
B)   They can recognize more than one epitope.
C)   The process used to produce the antibodies provides unlimited supplies.
D)   They can be generated without using animals.E) B) and D)
F) All of the above

Question 14

During the production of polyclonal antibodies, where are the antibodies isolated from?

Accepted Answer

A)   an immortalized tumor cell line
B)   liver cells
C)   spleen cells
D)   blood cellsE) B) and D)
F) None of the above

Question 15

Describe how an ELISA detects antigenic proteins.

Accepted Answer

ELISA uses a "sandwich" method where a p...

Question 16

Which technique uses highly specific monoclonal antibodies in a high-throughput form to detect small amounts of antigen?

Accepted Answer

A)   immunofluorescence
B)   Western blot
C)   SDS-PAGE gel
D)   ELISAE) A) and D)
F) C) and D)

Question 17

How can the mass of an unknown protein can be determined using gel electrophoresis?

Accepted Answer

The mass of an unknown protein can be de...

Question 18

The specific sites on the antibody that interacts with the antigen are

Accepted Answer

A)   epitopes.
B)   immunoglobin light chains.
C)   immunoglobin heavy chains.
D)   variable-domain amino acid residues.E) B) and D)
F) A) and D)

Question 19

Which type of chromatography separates proteins using specific binding properties?

Accepted Answer

A)   affinity chromatography
B)   gel filtration chromatography
C)   size exclusion chromatography
D)   high-performance liquid chromatographyE) A) and D)
F) C) and D)

Question 20

Calculate the purification of the target protein when there is a 30% decrease in activity and a 55% decrease in total protein after centrifugation.

Accepted Answer

A)   0.55-fold
B)   0.64-fold
C)   1.6-fold
D)   1.8-foldE) A) and C)
F) None of the above

Exam 5: Methods in Protein Biochemistry

What is the advantage of using polyclonal antibodies compared with monoclonal antibodies?

Correct Answer
verified

When preparing to isolate proteins from plant cells, the first step in preparing the cell homogenate would be

Correct Answer
verified

How does the directionality of synthesis differ with in vivo polypeptide synthesis compared to solid-phase peptide synthesis?

Correct Answer
verified
In in vivo polypeptide synthesis, amino ...
View Answer

Why is X-ray crystallography more commonly used than NMR spectroscopy when determining protein structure?

Correct Answer
verified
NMR is limited to proteins that are less...
View Answer

How can the mass of an unknown protein can be determined using gel electrophoresis?

Correct Answer
verified
The mass of an unknown protein can be de...
View Answer

Which type of chromatography separates proteins using specific binding properties?

Correct Answer
verified

Calculate the purification of the target protein when there is a 30% decrease in activity and a 55% decrease in total protein after centrifugation.

Correct Answer
verified

After centrifugation, the purity of the protein is determined by

Correct Answer
verified

In an ELISA, the capture antibody is a antibody.

Correct Answer
verified

Correct Answer
verified

In monoclonal antibody generation, the cells that produce the antibody in the animal are located in cells.

Correct Answer
verified

During the production of polyclonal antibodies, where are the antibodies isolated from?

Correct Answer
verified

Which technique uses highly specific monoclonal antibodies in a high-throughput form to detect small amounts of antigen?

Correct Answer
verified

In isoelectric focusing, a protein with a pH below the pI would

Correct Answer
verified

Explain how protein samples that contain the antigenic protein are recognized using a Western blot.

Correct Answer
verified
The proteins are separated using gel ele...
View Answer

What is the advantage of using monoclonal antibodies compared with polyclonal antibodies?

Correct Answer
verified

The specific sites on the antibody that interacts with the antigen are

Correct Answer
verified

Describe how an ELISA detects antigenic proteins.

Correct Answer
verified
ELISA uses a "sandwich" method where a p...
View Answer

Why is protein purification often referred to as an art and a science?

Correct Answer
verified
There are between 10,000 and 100,000 dif...
View Answer

How does NMR spectroscopy differ from X-ray crystallography when determining the structure of a protein?

Correct Answer
verified
X-ray crystallography gives the atomic p...
View Answer

Exam 5: Methods in Protein Biochemistry

What is the advantage of using polyclonal antibodies compared with monoclonal antibodies?

Correct AnswerverifiedShow Answer

When preparing to isolate proteins from plant cells, the first step in preparing the cell homogenate would be

Correct AnswerverifiedShow Answer

How does the directionality of synthesis differ with in vivo polypeptide synthesis compared to solid-phase peptide synthesis?

Correct AnswerverifiedIn in vivo polypeptide synthesis, amino ...View AnswerShow Answer

Why is X-ray crystallography more commonly used than NMR spectroscopy when determining protein structure?

Correct AnswerverifiedNMR is limited to proteins that are less...View AnswerShow Answer

How can the mass of an unknown protein can be determined using gel electrophoresis?

Correct AnswerverifiedThe mass of an unknown protein can be de...View AnswerShow Answer

Which type of chromatography separates proteins using specific binding properties?

Correct AnswerverifiedShow Answer

Calculate the purification of the target protein when there is a 30% decrease in activity and a 55% decrease in total protein after centrifugation.

Correct AnswerverifiedShow Answer

After centrifugation, the purity of the protein is determined by

Correct AnswerverifiedShow Answer

In an ELISA, the capture antibody is a antibody.

Correct AnswerverifiedShow Answer

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions. Which fraction corresponds to the cytosol fraction?

Correct AnswerverifiedShow Answer

In monoclonal antibody generation, the cells that produce the antibody in the animal are located in cells.

Correct AnswerverifiedShow Answer

During the production of polyclonal antibodies, where are the antibodies isolated from?

Correct AnswerverifiedShow Answer

Which technique uses highly specific monoclonal antibodies in a high-throughput form to detect small amounts of antigen?

Correct AnswerverifiedShow Answer

In isoelectric focusing, a protein with a pH below the pI would

Correct AnswerverifiedShow Answer

Explain how protein samples that contain the antigenic protein are recognized using a Western blot.

Correct AnswerverifiedThe proteins are separated using gel ele...View AnswerShow Answer

What is the advantage of using monoclonal antibodies compared with polyclonal antibodies?

Correct AnswerverifiedShow Answer

The specific sites on the antibody that interacts with the antigen are

Correct AnswerverifiedShow Answer

Describe how an ELISA detects antigenic proteins.

Correct AnswerverifiedELISA uses a "sandwich" method where a p...View AnswerShow Answer

Why is protein purification often referred to as an art and a science?

Correct AnswerverifiedThere are between 10,000 and 100,000 dif...View AnswerShow Answer

How does NMR spectroscopy differ from X-ray crystallography when determining the structure of a protein?

Correct AnswerverifiedX-ray crystallography gives the atomic p...View AnswerShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
In in vivo polypeptide synthesis, amino ...
View Answer

Correct Answer
verified
NMR is limited to proteins that are less...
View Answer

Correct Answer
verified
The mass of an unknown protein can be de...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The proteins are separated using gel ele...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
ELISA uses a "sandwich" method where a p...
View Answer

Correct Answer
verified
There are between 10,000 and 100,000 dif...
View Answer

Correct Answer
verified
X-ray crystallography gives the atomic p...
View Answer